ABSTRACT
Objective: To study the chemical constituents of the aerial parts of Ligularia veitchiana. Methods: The isolation and purification were carried out by macroporous resin, silica gel column chromatography, Sephadex LH-20, and semi-preparative HPLC. Their structures were elucidated on the basis of physicochemical properties and spectroscopic data. Results: A total of 16 compounds were isolated and elucidated from the aerial parts of Ligularia veitchiana, their structures were identified as: 2-hydroxplatyphyllide (1), ligudentatin A (2), 2-metyoxy-platyphillide (3), 7-hydroxy-11-hydroperoxybisabol-2,9E-diene (4), bisabola-2,11-diene-7α,10ζ-diol (5), 8β,10β-dihydroxyeremophilenolide (6), 3β(ax)-hydroxyeremophillenolide (7), 6β-hydroxy- 8α-ethoxyeremophil-7(11)-en-12,8β-olide (8), 3β,28-dibydroxylup-20(29)-ene (9), oleanolic acid (10), 3β-hydroxyolean-12-en-27- oic acid (11), ursolic acid (12), coniferyl ferulate (13), 4-O-Geranyl-sinapyl alcohol (14), 4-O-geranyl-coniferyl alcohol (15), auraptene (16). Conclusion: All compounds are isolated from Ligularia veitchiana for the first time. Compounds 4, 5, 7, 8, 11 are isolated from the genus Ligularia for the first time.
ABSTRACT
Objective: In this study, the feasibility of hot air-microwave combination drying method for processing Angelicae Sinensis Radix (ASR) was investigated and evaluated. Methods: Hot air temperature, drying time, microwave electric current, and microwave time were taken into consideration. The combined drying method was optimized based on the standards of ASR documented in Chinese Pharmacopoeia (2015 version). After that, microorganisms, heavy metals, harmful elements, and active ingredients (ferulic acid, coniferyl ferulate, senkyunolide I, senkyunolide H, senkyunolide A, E-ligustilide, Z-ligustilide, n-butylidenephthalide, riligustilide, and levistilide A) were detected to evaluate the quality of processed samples. Results: The optimal method was that samples were dried in hot air (70℃) for 20 h, then heated in microwave (100 mA) for 6 min. The number of microorganisms in processed samples equaled to sulfur-fumigated samples; Heavy metals and harmful elements were qualified after this processing; The new drying method for processing ASR had no significant effect on the active ingredient. Conclusion: After this process, the physical and chemical indicators of ASR are in line with the criteria of Chinese Pharmacopoeia. This hot air-microwave combination drying method should be a fast drying method for post-harvest handling of ASR.
ABSTRACT
Objective: This current study focused on the identification of active constituents from Angelica Sinensis Radix in Xiaoyao Powder based on UPLC-PDA-guided isolation technique. Methods: The UPLC-PDA chromatogram of Xiaoyao Powder was compared with that of Angelica Sinensis Radix. The relative retention time of each peak and the Uhraviolet spectra provided by PDA were used in the analyses. The constituents were isolated from Angelica Sinensis Radix under the guidance of UPLC-PDA investigation. The structures of the isolates were elucidated by NMR techniques. The antidepression effect was evaluated on glutamate-induced neurons. Results: Five marker peaks of Xiaoyao Powder fingerprint belonged to Angelica Sinensis Radix and they were determined as coniferyl ferulate (1), E-butylidenephthalide (2), ligustilide (3), Z-butylidenephthalide (4), and 14-acetoxy-12-senecioyloxytetradeca-2E,8E,10E-trien-4,6-diyn-1-ol (5). Compound 5 was isolated from the plants in Umbelliferae for the first time. The treatment with compounds 1, 3, and 4 could protect PC12 and SH-SY5Y cells from glutamate-induced cytotoxicity. Antidepression bioactivity of compound 1 was first investigated. Conclusion: UPLC-PDA-guided isolation technique is confirmed to be a rapid and accurate method to identify the main active constituents from Angelica Sinensis Radix in Xiaoyao Powder.